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Genetics and Cell Biology
of Basidiomycetes VII
May 28-June1, 2008
Southeast Missouri St. U.
Cape Girardeau, MO
Call For Papers
Closed
This is the call
for papers for the VIIth Genetics and Cell Biology of Basidiomycetes
conference. We anticipate time for 36 oral
presentations, with the remainder to be given as posters.
Don't forget to register for
the conference, using the separate link at the bottom of the
page.
Submission
Send your
abstract to
GCBBmeeting@list.semo.edu as an attachment in the form of
a Microsoft Word document. Deadline for submission is March
31,
2008. Please indicate in the body of your email (not in the attached
abstract) whether you prefer to give an oral presentation or to bring
a poster. The program committee will attempt to honor such preferences
where possible.
Title
Write the title in 12 point bold Times New Roman
or similar font (with serifs), centered on the page. Titles should be
under 100 characters, including punctuation and spaces. Italicize
scientific names of genera and species.
Authors
In 12
point (not bold) Times New Roman or similar font, left justified, list
authors' names in upper and lower case, with initials before the
surname. Separate authors' names with commas. Underline the
presenter's name. On separate lines below the author list, give the
postal address and the email address of each author. Use superscript
numbers to identify author affiliations.
Body
of abstract
The
abstract should be written in English, in 12 point (not bold) Times
New Roman or similar font. It should be a single paragraph, not
exceeding 350 words, and should be left-justified.
Example: |
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Expressed sequence tags
from Schizophyllum commune grown under different nitrogen
conditions
Guettler S1, Lucchese S1, Honass L2
1Southeast Missouri St. U., Cape Girardeau, MO 63701
USA.
2Pennsylvania St. U., University Park, PA 16802 USA.
Schizophyllum commune is a common wood-decaying
basidiomycete. Growing on a nitrogen-poor medium, it establishes
recycling mechanisms to provide new mycelia with a sufficient supply
of amino acids. We analyzed the gene expression of Schizophyllum
commune under three different nitrogen availability conditions
utilizing previously prepared cDNA libraries in the lambda-Zap vector
(Stratagene): MIN (from mycelia grown on minimal medium), 6HR (six
hours after transfer to low-nitrogen medium), and MO1 (twelve hours
after transfer). Expressed sequence tags (ESTs) are short cDNA
sequences that represent gene expression in organisms under distinct
conditions. We used two different approaches to obtain ESTs: single
clone excision and mass excision. In single clone excision, clones
were selected at the vector stage and excised from lambda-Zap vector
to generate pBluescript phagemids, which were amplified in E. coli,
isolated, and sequenced. In mass excision, phagemids were prepared
from the whole cDNA library, and the clone selection step was
performed after transformation of E. coli with the phagemids.
The cDNA inserts were sequenced and compared to nucleotide sequences
in GenBank to determine their identity. The obtained ESTs were
submitted to the GenBank database. They include fragments of genes
similar to sequences encoding the HECT domain of ubiquitin-ligase (E3)
enzyme, glucan synthase, HSP90-associated chaperone, cell division
control protein 2, and ubiquitin carboxyl-terminal hydrolase. The
large number of identifiable genes isolated so far suggests that this
approach may help reveal the mechanism of response to nitrogen
limitation in Schizophyllum commune.
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